Evolution of Taste: Early Sharks were Able to Perceive Bitter Substances
Published:10 Jan.2024    Source:University of Cologne
A research team from the University of Cologne, in collaboration with colleagues from the Leibniz Institute for Food Systems Biology in Freising, has discovered a receptor for bitter taste in twelve different cartilaginous fish (sharks and rays). The receptor belongs to the so-called taste receptors type 2 (T2R), which also make humans perceive bitter and potentially toxic foods. Until now, it was assumed that such receptors only occur in bony vertebrates. The work was published under the title 'A singular shark bitter taste receptor provides insights into the evolution of bitter taste perception' in the journal Proceedings of the National Academy of Sciences (PNAS).
Twelve out of seventeen cartilaginous fish genomes studied contained genes for the taste receptors type 2, with only one T2R gene present in each species. The researchers named this single gene T2R1. The fact that only a single T2R gene was found suggests that it is the original form of these bitter taste receptors, which was not altered by gene duplication and subsequent different specialization of the resulting receptors.

'These findings give us new insights into the evolution of these receptors: We can look back almost 500 million years on the molecular and functional origin of an entire family of bitter taste receptors. Because that is how old the last common ancestor of cartilage and bony fish is,' says Sigrun Korsching. The authors have also introduced the T2R1 gene of the bamboo shark (C. plagiosum) and the catshark (S. canicula) into immortalized cell lines. The results showed that both sharks can taste bitter substances also perceived by humans, such as colchicine or bile acid. A screening of ninety-four human bitter substances identified eleven substances that could also activate the sharks' receptors. Some of these eleven substances also activate the bitter taste receptors of the 'living fossil' coelacanth (Latimeria chalumnae), an ancient species of bony fish, as the authors have shown in a previous study. Sigrun Korsching summarizes that 'the extent to which this function has been conserved is astonishing, i.e. through the entire evolution of vertebrates.'