Research Article
Some Metal Elements Concentration in Malletia cumingii (Hanley, 1860) (Bivalvia) in Okpon River, Cross River State, Nigeria 
,
Eyom Ibiam Asira 
Author Correspondence author
International Journal of Aquaculture, 2017, Vol. 7, No. 25 doi: 10.5376/ija.2017.07.0025
Received: 16 Aug., 2017 Accepted: 06 Sep., 2017 Published: 31 Dec., 2017
Ada F.B., and Asira E.I., 2017, Some metal elements concentration in Malletia cumingii (Hanley, 1860) (Bivalvia) in Okpon River, Cross River State, Nigeria, International Journal of Aquaculture, 7(25): 166-173 (doi: 10.5376/ija.2017.07.0025)
Molluscs are important food item all over the globe. They have been harvested from the wild and some cultured for food; such that 20% of aquatic animal protein comes from molluscs. They have also been widely used as indicator of pollution. On the other hand, many are intermediate hosts of parasitic disease of many animals and man. Malletia cumingii is usually harvested in Okpon River, a tributary of Cross River in Cross River State of Nigeria. Because of the value of Molluscs as indicator of pollution, this species was analyzed to ascertain the metal elements concentration levels for reason that some of these metals are toxic to man. Samples were collected from three locations along the River. Sampling was done two times to include the two main seasons of the year (dry and wet seasons). The samples were oven-dried (MAMMART) at 100°C and were pounded in mortar to powder form. Metal concentrations were measured using flame photometer (RP 7) and atomic absorption spectro-photometer. Metals analyzed included Copper, Cadmium, Chromium, Iron, Manganese, Nickel, Lead, Zinc, Calcium and Magnesium at different wavelengths. Results showed that metal concentration was increasing from the upper reaches of the river downward except zinc. It was suggested that zinc have a point source in the upper reaches while others metals were added to the water body by anthropogenic activities. However, the concentration of the metals did not reach toxic level in the molluscs for human consumption.
1 Background
The study of molluscs is always an important one as molluscs are used as human, aquatic and many terrestrial animals’ food (Gupta and Gupta, 2006). Because of their importance in human food, they are used in Aquaculture to create a net gain in protein to help alleviate the fishing pressure on feral fin fish. Nine million, one hundred Metric tonnes (22 percent) of Aquaculture products in 2014 A.D. were molluscs (FAO, 2016). Unlike carnivorous fish, which need a fish-based food, molluscs feed on diet based on plant materials. Molluscs feed both in natural and artificial environments. They are less expensive because they are of low trophic level (Simpkins and Williams, 1989; Okon and Ibom, 2012). Sequel to this, species like Pacific oyster (Crassostrea gigas), Japanese carpet shell (Ruditapes philippinarum), Yesso scallop (Patinopecten yessoensis), blue mussel (Mytilus edulis) and the blood cockle (Anadara granosa have been cultured). Contribution of molluscs to world food fish Aquaculture in 2013 was 20.10% by weight (15.5 million metric tons) and 13.80 % by money value (20.7 USD) (FAO, 2015).
Many serve as intermediate hosts of parasitic helminthes (David, 1994; Ntonifor and Ajayi, 2007; Abe, 2010; Kalu et al., 2012). They are also identified as the most appropriate group of organisms to be used as environmental bio indicators because of the following qualities. They are cosmopolitan in distribution, individuals have limited mobility, exhibit wide variation in life cycle strategies such that a particular strategy can be monitored, their sizes are large enough, absence of external and internal skeleton bring them closer to their environment and have limited ability to excrete pollutants directly via their kidney or other excretory tissues, they are sensitive to environmental chemicals, simple and well and well known internal organisation an quantities of the pollutants are measurable in their tissues, organs or whole organism (Oehlman and Schutle-Oehlman, 2003).
Modification or changes in environment is a continuous phenomenon. Altering their habitat affects survival (Ayotunde and Ada, 2013). Direct destruction of some of these habitats because of agricultural and urban development and habitat transformation resulting from dam construction and human settlements are important causes of molluscs’ population declines in many areas (Hunters et al., 1976; Yang et al., 2013). Most freshwater molluscs species are highly sensitive to water quality because of their permeable skins and because they need a good oxygen supply. Larval stages of unionids require fish as a host for development. As fish stock become scanty, the parasitic larval stages of the molluscs lack host to complete their development. It therefore means that loss of fin fish stock leads to loss of bivalve requiring the fin fish their development in an area. Continuing loss of molluscs diversity is detrimental, not only to ecosystems around the world, but in the long run, to the welfare of mankind itself (Svobodová et al., 1993). Indeed, molluscs are crucial to the integrity of ecosystems, the evaluation of environmental health, and human well-being.
Some heavy metals have beneficial functions to the bodies of organisms while others are not and can be highly toxic. They accumulate in the body tissue of organisms because they are rarely broken down (Ekpo et al., 2008). However, large quantity of heavy metals and derivatives are discharged by industrial plants and municipal sewage treatment plants into the aquatic ecosystems. They may also get into the water from polluted runoff in urban and agricultural areas (Ayotunde et al., 2012).
Okpon River serves as a major source of drinking water and site of fishing activities. There are many fishing landing sites along the river. These include: Ekurri beach, Okpon beach and Nsin beach. Where the river transverse villages, there are sand mining activities. This forms a big industry in Ochon where the river transverses Calabar-Ikom high way, English Sand beach and Men sand beach are equally found in Ochon. Other sand beaches include those in Ekurri (Ekurri sand beach) and Nsin (Nsin sand beach), Onyen Okpon (Monkey Agwu Okpa sand beaches). This equally provides livelihood for the inhabitants. There is relatively a high population density along the river due to its reach resources (Ako and Salihu, 2004; Study.com, 2016). Logging activities have been going on. As Rainforest Movement (2016) wrote ‘Logging has significantly reduced animal habitat, shrinking the animal populations that serve as a traditional source of protein in Cross River State. Meat obtained from feral animals commonly called bush meat that was once plentiful is now scarce (pers. observ.). The plundering of trees which provide shelter has left whole areas without windbreakers or sufficient trees to check the devastating rainstorms. Thus, the roofs of houses are often blown off by the slightest rainstorm.’ Because of high anthropogenic activities, natural phenomenon like weathering and seapage of materials from surrounding basements, there is likely to be inflow of metals into the river. In addition to indirect feeding of aquatic systems with contaminants, humans feed the system with metal containing items like personal care products (Schueler, 2000; Burton and Pitt, 2007). Apart from metal pollution, climate change which is presently much talked about is likely to affect the ecology of the river.
Due to the toxicity level of heavy metals concentrations in aquatic food items such as fish species, they have received considerable attention in different places (Mohamed and Gad, 2008; Klavins et al., 2009; Ayotunde et al., 2012; Ekpenyong et al., 2015). Many research interests have been generated on the integrity of aquatic environment especially as it concerns heavy metal pollution (Rashed, 2001; Tawari-Fufeyin and Ekaye, 2007; Yilmag, 2007; Yilmag et al., 2009). As Elder and Collins (1991) pointed concentration of metals in an organisms cannot be extrapolated from place to place due to differences in individual organisms and environmental characters, we work to investigate the concentration of some metals in Malletia cumingii in Okpon River.
2 Materials and Methods
2.1 Study area
Okpon River is a tributary of Cross River. It has its source from Etung Local Government Area of Cross River State Hills. Okpon River flows from Ekurri in Etung Local Government Area and empties into Cross River. The Okpon River is located in the southern part of Nigeria latitude 4°25”-7°0” North and longitude 7°15”-9°30” East. The climate of the area is characterized by two seasons dry and raining. The raining season is (May-October) with higher precipitation, while the dry season is (November-April) with low precipitation (Freund et al., 1995).
The vegetation of the river is made up of dense growth of tall trees made of thick foliage. The foliage reduces the amount of sunlight that gets into the river that in turn plays a part in the distribution of types of organisms in the river ecosystem Due to the fertility of the Okpon River Basin; there is a high concentration of human population along the River. Fishing activity includes the harvesting of Mellitia cumingii which is a source of animal protein for the local population.
Samples were collected from three locations along the River as indicated in the map (Figure 1). Sampling was done two times (in January and July of 2006) to include the two main seasons of the year (dry and wet seasons). Concentrations were analyzed and average of both the dry and wet seasons found. The specimens were dissected according to guides from Olayemi et al. (2012) to remove the tissues. These were preserved in pure H2SO4 waiting for laboratory analysis of the metals (Khadse, 2010).
|
Figure 1 Map of Cross River State showing the location of the sampling stations as bold crosses (Ekurri, Nsin and Ochon) along the river |
.png)
The tissues were removed from preservative and soaked in water for four hours. These were washed in deionized water repeatedly to rinse off the preservative. The samples were oven-dried (MAMMART) at 100°C and were pounded in mortar to powder form. One gram of each sample was digested in 15 ml of nitric acid and 5 ml of perchloric acid. The mixture was heated on a hot plate until it became solid. This was digested to a clear liquid and made up to 100 ml using deionized water. Metal concentrations were measure using flame photometer (RP 7) and atomic absorption spectro-photometer. Metals analyzed included Copper, Cadmium, Chromium, Iron, Manganese, Nickel, Lead, Zinc, Calcium and Magnesium at different wavelengths as shown in Table 1.
|
Table 1 Wavelengths use in measuring light absorbance for various metals at different lamp apertures and instrument used Note: AAS = Atomic absorption spectrophotometer; FP = Flame photometer |
.png)
3 Results
Concentrations of metals in all tissues combined for a particular station were displayed in Table 2. Metals that were different in their concentration among the stations included Manganese (Mn), Lead (Pb), Zinc (Zn) and Calcium (Ca). Iron (Fe), Cadmium (Cd), copper (Cu) and Magnesium (Mg) were not significantly different in concentration among stations. Means which carry the same letters were statistically the same while those with different letters were statistically different.
|
Table 2 Show the mean values of the metals at the various stations in Malletia cumingii from Okpon River |
.png)
Figure 2; Figure 3; Figure 4 show the concentrations of the different elements in the various tissues namely mantle, abductor muscle foot and gills in different stations. In Figure 2 it was observed that manganese had a high absolute concentration value in the mantle compared to other elements. Manganese and zinc were comparatively higher than other elements in the mantle in the different stations, while calcium was higher than other elements in different tissues and stations. Figure 5 is a four axis graph which shows that calcium was the most highly concentrated as it is furthest apart from the origin of the graph. This was followed by magnesium and manganese.
|
Figure 2 Metals elements concentrations in the tissues of Malletia cumingii in station A (Ekurri). All the elements were not significantly different (α = 0.05) in concentration except Magnesium whose concentration was lower in the mantle compared to other tissues. It is also observed that Calcium was higher than any other metal followed by Magnesium |
.png)
|
Figure 3 Metals elements concentrations in the tissues of Malletia cumingii in station B (Ochon). Manganese, Cadmium, Zinc, Calcium and Magnesium were not statistically different between one organ and the other at (α = 0.05), but Iron, Lead and Copper were differentially concentrated among the tissues |
.png)
|
Figure 4 Metals elements concentrations in the tissues of Malletia cumingii in station B (Nsin). All the elements except lead and copper were not significantly different in concentration in the various tissues; Lead and Copper were less concentrated in the abductor muscle. Copper was least concentrated in the foot compared to all other tissues (α = 0.05) |
.png)
|
Figure 5 A graph of metal elements concentration in the tissues Malletia cumingii obtained from the three sample stations of Okpon River, Cross River State, Nigeria. Calcium was the most highly concentrated followed by Lead |
.png)
4 Discussion
Magnesium and Calcium were higher in concentration than other elements in station A. All others were not statistically different in concentration. Magnesium and Calcium are usually part of biological systems. These are usually not grouped as heavy metals as their relative mass is not greater than five times the weight of water (Glanze, 1996; Duruibe et al., 2007). Magnesium is known to regulate the functions of enzymes. It is co-factor of enzymes that break down fat and glucose and functions in the production of proteins. Since it is important in the formation of energy currency, ATP (Adenosine Triphosphate) it could be regarded as a source of fuel. Its role in the proper functioning of sodium-potassium pump makes it important in maintenance of electrolyte balance in the bodies of organisms (Byjus, 2015). Calcium is abundant in bones of organisms. Bivalves have no bones. Its exoskeleton (the shell) is highly impregnated with calcium (Smith, 1986). Its high concentration was not a surprise therefore because it is needed for further formation (growth) of the shell. Apart from shell formation, it is important in muscle contraction and nervous coordination (Pleshchitser, 1958; Byjus, 2015). Oehlman and Schulte-Oehlman (2003) pointed that molluscs are good indicators of metal availability as they are one group of animals with metallothioneins, which are low-weight molecule protein swith high cysteine content and having high affinity for absorbing trace elements.
Metals concentrations above normal concentrations constitute pollution. Apart from pollutants entering the water systems through weathering (Idodo and Oronseye, 2006), anthropogenic sources are the reasons for entry of pollutants into water (Ogbeibu and Ezeunara, 2002). For instance, the concentration of Lead, increased from station A to C. Such increases could be attributed to increasing anthropogenic impact. For instance, Lead is a component of fuel used in automobiles and light generators. The number of light generators and automobiles are adding as one more from the upper reaches of the river to the lower reaches. If the Lead is not biodegraded or removed by chemical reaction, or these processes do not remove it more than it is added, the concentration is expected to increase downward as observed in Table 2. We may argue that it is because settlement increases and adds downward from the upper part of the river downwards. It is not every pollutant or element that increases in concentration down the river. Some elements could be released from a source and may be consumed by organics or even chemical reactions as it moves down the river. Such was observed in Table 2 for Manganese. There may be a point source pollution for this element in the upper reaches of the river, which may be absorbed, or removed by chemical binding to other substances.
Many organisms have been used as bioindicators of aquatic environmental pollution. As Oehlman and Schulte-Oehlman (2003) posited, organisms which have limited range of mobility are good bioindicators. Malletia cumingii is a benthic bivalve with proximity to bottom sand. Metal elements load or metal element concentrations in the environment can be estimated from this kind of species. Similarly, it is the opinion of Khatri and Tyagi (2015) that through the application of Bioindicators we can predict the natural state of a certain region or the level/degree of contamination. It is also pointed out that the concentration of metals in an organism is not necessarily an indication of the level of concentration in the water column. According to Elder and Collins (1991), bioaccumulation and toxicity are extremely situation dependent; therefore, concentration levels should not be extrapolated from one particular area to other situations where the biological species or environmental conditions are different. That even within one species, individual characteristics such as size, life stage, sex, and genotype can have significant effects on responses to contaminants.
The quantity of metals absorbed and maintained in an organisms’ body depends on a lot of factors as was explained by Cheng (1987), Elder and Collins (1991), Ayotunde et al (2012) and Oehlman and Schulte-Oehlman (2003) to include; concentrations and chemical characteristics of water, ecological needs, metabolism and feeding patterns of organism tolerance, size, life stages, species, organic substances, pH, temperature, alkalinity, hardness, inorganic ligands, interactions, sediments and season. These factors can equally alter metal toxicity in the aquatic environment substantially by causing attenuating effect.
Abe E.M., 2010, Geospatial distribution of fresh water snails intermediate hosts of parasites in Abeokuta Area of Ogun State, Nigeria. A PhD Thesis submitted to the Department of Biological Sciences, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
Ako P.A., and Salihu S.O., 2004, Studies on some major and trace metals in smoked and oven-dried fish, Journal of Applied Science and Environmental Managment, 8: 5-9
http://bioline.utsc.utoronto.ca/archive/00003055/01/ja04016.pdf
Ayotunde E.O., and Ada F.B., 2013, Conservation of biodiversity in Central Cross River State, Nigeria: he role of indigenous communities. Research Journal of Fisheries and Hydrobiology, 8(2): 20-26
Ayotunde E.O., Offem B.O., and Ada F.B., 2012, Heavy metal profile of water, sediment and freshwater cat fish, Chrysichthys nigrodigitatus (Siluriformes: Bagridae) of Cross River, Nigeria, Rev. Biol. Trop. International Journal of Tropical Biology, 60(3): 1289-1301
http://www.scielo.sa.cr/pdf/rbt/v60n3/a27v60n3.pdf
Burton B.A., and Pitt R., 2007, Stormwater Effects handbook: a toolbox for watershed managers, scientists, and engineers
Byjus, 2015, Biological importance of calcium and magnesium
David S.B., 1994, Fresh water snails of Africa and their medicinal importance CRC Press.com, pp.607
Duruibe J.O., Ogwuegbu M.O.C., and Egwurugwu J.N., 2007, Heavy metal pollution and human biotoxic effects, International Journal of Physical Sciences, 2(5): 112-118
http://www.academicjournals.org/article/article1380209337_Duruibe%20et%20al.pdf
Ekpenyong E., Ada F.B., Udofia U.U., and Etangetuk N.A., 2015, Assessment of metal concentrations in periwinkle (Tympanotonus fuscatus) and bivalve (Egeria radiata) from the Cross River, South Eastern Nigeria, Journal of Biopesticides and Environment 2 (1-2): 1-6
Ekpo K.E., Asia I.O., Amayo K.O., and Jegede D.A., 2008, Determination of lead, cadmium and mercury in surrounding water and organs of some species of fish from Ikpoba river in Benin city, Nigeria, International Journal of Phyiological Science, 3(11): 289-292
http://www.academicjournals.org/app/webroot/article/article1380375553_Ekpo%20et%20al..pdf
Elder J.F., and Collins J.J., 1991, Fresh water molluscs as indicators of bio availability and toxicity of metals in surface water system, Review in environmental contamination and toxicology, 122; 37-79
https://doi.org/10.1007/978-1-4612-3198-1_2
PMid:1771274
FAO, 2015, Global Aquaculture production statistics database summary information, Food and Agricultural Organization of the United Nations,
http://www.fao.org/fishery/statistics/en
FAO, 2016, FAO yearbook, Fishery and Aquaculture Statistics, 2014, Rome, Italy
Glanze W.D., 1996, Mosby Medical Encyclopedia, Revised Edition, C.V. Mosby, St. Louis, MO
Gupta S.K., and Gupta P.C., 2006, General and Applied Ichthyology (Fish and Fisheries), S. Chand and Company Limited, Ram Nagar, New Delhi, pp.1133
Hunters G.W., Swartzwelder J.C., and Clyde D.F., 1976, Tropical Medicine, 5th ed. W.B. Saunders Co. Philadelphia, pp.543-568
Idodo-Umeh G., and Oronseye J.A.O., 2006, Heavy Metal Pollution in Ololomoro, Niger Delta, Nigeria. Journal of Fisheries, 2/3(2): 267-279
Khadse G.K., 2010, Collection and preservation of samples and field analysis presented at: Training Programme on QAQC in Water Quality Monitoring, Assessment, Oct. 21-22
http://mpcb.gov.in/envtdata/VNIT%20water%20-sampling.pdf
Khatri N., and Tyagi S., 2015, Influences of natural and anthropogenic factors on surface and groundwater quality in rural and urban areas, Frontier of Life Science, 8(1): 23-39
https://doi.org/10.1080/21553769.2014.933716
Klavins M., Potaporics O., Rodinov V., 2009, Heavy metals in fish from lakes in Latvia: concentrations and trends of changes, Bulletin of Environmental Contamination and Toxicology, 82:96-100
https://doi.org/10.1007/s00128-008-9510-x
PMid:18709332
Mohamed E.A.S., and Gad N.S., 2008, Environmental pollution - inducesd biochemical changes in tissues of Tilapia Zilli, Solea. vulganis and Mugil capito from Lake Oarun, Egypt, Global veterinaria, 2(6): 327-336
Ngele K.K., Kalu K.E.O., Ukwe M.C., and Onyeuwu C.N., 2012, A survey of freshwater snails: the intermediate hosts of schistosomiasis in Bende L.G.A., Abia State Nigeria International Journal Science and Nature 3(4): 879-882
Ntonifor H.N., and Ajayi J.A., 2007, Studies on the ecology and distribution of some medically important freshwater snail species in Bauchi State, Nigeria, International Journal of Biological and Chemical Sciences 1(2): 121-127
https://doi.org/10.4314/ijbcs.v1i2.39681
Oehlman J., and Schulte-Oehlman S., 2003, Molluscs as bioindicators
https://doi.org/10.1016/S0927-5215(03)80147-9
Ogbeibu A.E., and Ezeunara P.U., 2002, Ecological impact of brewery effluent on Ikpoba river using the fish communities as bio indicators. J. Agua. Res. 17 (1): 35-44
https://doi.org/10.4314/jas.v17i1.19908
Okon B., and Ibom L.A., 2012, Snail breeding and snailery management, Freshdew publication, 13, Etta Agbor Avenue, Calabar, pp.70
Olayemi I.K, Ayanwale A.V, Odeyemi O.M., and Mohammed A.Z.A., 2012, Comparative Study of the Anatomy of Two West African Edible Bivalves, Aspatharia Sinuata (Mutellidae: Unionacea) and Egeria Radiata (Donacidae: Tellinacea), International Journal of Applied Biological Research, 4(1&2): 114-120
Pleshchitser A.I., 1958, Biological role of magnesium
http://clinchem.aaccjnls.org/content/4/6/429 Accessed 4/3/2017
Rainforest Movement, 2016, Nigeria: Either legal or illegal, commercial logging in Cross River State forest.
http://wrm.org.uy/oldsite/bulletin/86/Nigeria.html Accessed 2/19/2016
Rashed M.N., 2001, Monitoring of Environmental heavy metals in fish from Nasser Lake, Environment International, 8: 27-33
https://doi.org/10.1016/S0160-4120(01)00050-2
Schueler T.R., 2000, Cars are leading source of metal loads in California, The Practice of Watershed Protection, Ellicott City
PMCid: PMC17207
Simpkins J., and Wlliams J.I., 1989, Advanced Biology, 3rd edition, English Language Book Society/Unwin Hyman Ltd. Broadwick Street, London, pp.760
PMCid: PMC2626097
Smith R.L., 1986, Elements of Ecology, Second edition Harper and Row publisher Inc.10 East 53d Street, New York, USA pp.477
Study.com, 2016, Environmental Influences on Human Settlements and Activities
Svobodová Z., Lloyd R., Máchová J., and Vykusová B., 1993, Water quality and fish health, EIFAC Technical Paper. No. 54, FAO, Rome, pp.59
Tawari-Fufeyin P., and Ekaye S.A., 2007, Fish species as indicator of pollution in Ikpoba River, Benin City, Nigeria, Review of Fish Biology and Fisheries, 17: 21-30
https://doi.org/10.1007/s11160-006-9015-9
Wang W., 1987, Factors affecting metal toxicity to and accumulation by aquatic organisms Overview
https://doi.org/10.1016/0160-4120(87)90006-7
Yang T., Wu Z., and Lun Z., 2013, The Apple Snail Pomacea canaliculata, a Novel Vector of the Rat Lungworm, Angiostrongylus cantonensis: its Introduction, Spread, and Control in China Hawaii, Journal of Medical Public Health, 72(6 Suppl 2): 23-25
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689487
PMCID: PMC3689487
Yilmag F., 2009, The comparison of heavy metal concentrations in tissues of economically important fish from koycegiz lake - Mugla, Turkish Journal of Science and Technology, 4(1): 7-15
Yilmag F., Ozdemir N., Demirak A., and Tuna A., 2007, Heavy Metal levels in fish Species, Food chemistry, 100: 830-835
. PDF(356KB)
. FPDF
. HTML
. Online fPDF
Associated material
. Readers' comments
Other articles by authors
. Fidelis Bekeh Ada
. Eyom Ibiam Asira
Related articles
. Metal elements
. Malletia cumingii
. Okpon River
. Concentration
. Toxicity
. Cross River State
Tools
. Email to a friend
. Post a comment