Gut Associated Lactic Acid Bacteria Isolated from the Estuarine Fish  Mugil cephalus : Molecular Diversity and Antibacterial Activities against Pathogens

Shubhankar Ghosh<sup>1</sup>; Einar Ringo<sup>2</sup>; A. Deborah Gnana Selvam<sup>1</sup>; K. M. Mujeeb Rahiman<sup>1</sup>; Naveen Sathyan<sup>1</sup>; Nifty John<sup>1</sup>; A. A. M. Hatha<sup>1</sup>

Gut Associated Lactic Acid Bacteria Isolated from the Estuarine Fish Mugil cephalus: Molecular Diversity and Antibacterial Activities against Pathogens

Shubhankar Ghosh¹

, Einar Ringo²

, A. Deborah Gnana Selvam¹

, K. M. Mujeeb Rahiman¹

, Naveen Sathyan¹

, Nifty John¹

, A. A. M. Hatha¹

1. Dept. of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine arts Avenue, Cochin- 682016, India
2. Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, University of Tromsø, N-9037, Brevikia, Norway

Author

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International Journal of Aquaculture, 2014, Vol. 4, No. 1 doi: 10.5376/ija.2014.04.0001
Received: 11 Nov., 2013 Accepted: 18 Dec., 2013 Published: 22 Jan., 2014

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Preferred citation for this article:

Ghosh et al., 2014, Gut Associated Lactic Acid Bacteria Isolated from the Estuarine Fish Mugil cephalus: Molecular Diversity and Antibacterial Activities against Pathogens, International Journal of Aquaculture, Vol.4, No.01: 1-11 (doi: 10.5376/ija.2014.04.0001)

Abstract

In the present study we address the issue on gut associated lactic acid bacteria (LAB) isolated from the intestine of estuarine fish Mugil cephalus using de Man Rogossa and Sharpe (MRS) agar. LAB isolates were identified biochemically and screened for their ability to inhibit in vitro growth of various fish, shrimp and human pathogens. Most of the LAB isolates displayed an improved antagonism against fish pathogens compared to shrimp and human pathogens. Selected representative strains displaying high antibacterial activity were identified using 16S rRNA gene sequence analysis. Of the selected strains Lactobacillus brevis was the most predominant. Four other species of Lactobacillus, Enterobacter hormaechei and Enterobacter ludwigii were also identified. It was also observed that even among same species, considerable diversity with respect to substrate utilization persisted. Considering the euryhaline nature of grey mullet (Mugil cephalus), the LAB isolated from the gut possessed good tolerance to varying salt concentrations. This finding merits further investigation to evaluate whether the isolated LAB could be used as probiotics in various fresh and sea water aquaculture.

Keywords

Probiotics; Antibiotic resistance; Lactobacilli; Molecular diversity

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