Lindernia antipoda L. (Alston) is one of the valuable aquatic ornamental plants which have a bright potential in the aquarium trade. In the present study a protocol for rapid shoot multiplication and in vitro flowering from axillary bud was developed. Half MS media with 1 mg/L benzylaminopurine (BAP) was the found to be the best medium for culture initiation and establishment. The effective medium for shoot multiplication was MS+1 mg/L BAP+0.2 mg/L NAA. In this media, 85% of the cultures exhibited multiple shoot proliferation. The highest shoot proliferation and shoot length were also acquired in this media. Plant showed highest in vitro flowering in MS medium supplemented 1 mg/L BAP and 0.2 mg/L α-Naphthalene acetic acid (NAA). All concentrations of NAA induced rooting. Hardening of in vitro cultured plantlets were attained through polythene propagator system and 90% of the plants survived, and it was successfully transformed in to an aquarium.