Research Report

Analysis of SSR Information in Aquatic Plant Euryale ferox Salisb Transcriptome  

Bowen Xue , Fangfang Sun , Ronghua Zhou , Jun Xu , Yulai Yin
Suzhou Academy of Agricultural Sciences, Suzhou, 215000, China
Author    Correspondence author
International Journal of Aquaculture, 2023, Vol. 13, No. 2   doi: 10.5376/ija.2023.13.0002
Received: 07 Mar., 2023    Accepted: 16 Mar., 2023    Published: 13 Apr., 2023
© 2023 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Xue B.W., Sun F.F., Zhou R.H., Xu J., and Yin Y.L., 2023, Analysis of SSR information in aquatic plant Euryale ferox Salisb transcriptome, International Journal of Aquaculture, 13(2): 1-7 (doi: 10.5376/ija.2023.13.0002)

Abstract

In order to study the genetic diversity breeding of aquatic plant Euryale ferox Salisb, this paper developed Euryale ferox Salisb EST-SSR marker by transcriptome sequencing technology, and 86 829 Unigenes were screened. Then we got 9 640 SSR loci from transcriptome by using the software MISA, accounting for 11.10% of all the Unigenes, the average distributing distance is about 7.65 kb. The Di and Trinucleotide are the major repetitive types in Euryale ferox Salisb transcriptome, accounting for 61.88% and 21.55% of all the SSRs, respectively. There are 226 repeat motifs were detected, of which A/T, AG/CT and AAG/CTT were high frequency repeat motifs, accounting for 1.25%、50.87% and 6.51% of all repeat motifs, respectively. The SSR motif length is centered in 12~20 bp, while the number of SSR over 20 bp was 717, accounting for 7.44%. 11 pairs of SSR primers were successfully designed and screened. The results showed that the SSR loci of the Euryale ferox Salisb transcriptome were higher in frequency, rich in type, which could serve as foundation for the further development of SSR markers and genetic diversity.

Keywords
Euryale ferox Salisb; Transcriptome; SSR; Site information
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