Fadhil N.A. Alkanany , Satar A. Gmais , Anwar A. Maki , Asaad M.R. Altaee

Marine Science Center, University of Basra, Iraq
Author    Correspondence author
International Journal of Marine Science, 2017, Vol. 7, No. 42   doi: 10.5376/ijms.2017.07.0042
Received: 01 Sep., 2017    Accepted: 27 Sep., 2017    Published: 20 Oct., 2017

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Alkanany F.N.A., Gmais S.A., Maki A.A., and Altaee A.M.R., 2017, Estimation of bacterial biodegradability of PAH in Khor Al-Zubair channel, Southern Iraq, International Journal of Marine Science, 7(42): 399-410 (doi: 10.5376/ijms.2017.07.0042)

Four bacterial strains were isolated from Khor Al-Zubair channel, southern Iraq based on a high growth rate on crude oil and on hydrocarbon degradation ability. The strains were preliminarily identified based on morphological observation, and by the Vitek II system. The isolates were identified as Brevundimonas diminuta /vesicularis, Vibrio vulnificus, Sphingomonas paucimobilis and Ochrobactrum anthropic. However, the ability of these strains to utilize crude oil (0.25%; 0.5%; 1% and 2% v/v) varied both in rates of utilization and in growth profiles. The components of crude oil degraded by the isolates were determined by capillary gas chromatography. Sphingomonas paucimobilis and Vibrio vulnificus were the most effective bacteria degrade PAH on seventh day incubation, especially in concentration 2.0 mg/l (97.39% and 84.23%) respectively, meanwhile, Brevundimonas diminuta/ vesicularis was highly effective degrade PAH (73.41% and 62.08%) in concentration 1.0 and 0.5 mg/l, respectively. On the other hand, Ochrobactrum anthropi was the lowest in all concentrations.

Khor Al-Zubair; Bacteria; Vitek II system; Gas chromatography